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Histopathology / Cytopathology / Hematopathology / PLA Home Page |
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Assemble the aspirating equipment. If direct smears are to be made, label
the slides prior to the aspiration. With the target of aspiration
fixed with the non-dominant hand between the thumb and index
finger and the syringe or syringe pistol in the dominant hand,
the needle is placed against the skin. The needle should approach
the skin at approximately a 30-degree angle if the lesion is
very superficial. If the mass is deep, the needle should approach
the skin at a perpendicular angle. A quick motion should be used
in passing the needle through the skin. The needle is then advanced
through the subcutaneous tissue into the mass. If the mass is
small the needle should be aimed toward the center; if it is
large the needle should be aimed toward the periphery (because
the center of larger masses may be necrotic). A noticeable difference
in the consistency of the tissue should be noted when the needle
penetrates the mass. With the needle in the mass the needle tip
should be moved in short motions initially to loosen cells within
the mass. Negative pressure is then applied by pulling back on
the plunger of the syringe. When blood or material appears in
the hub of the needle the aspiration should be stopped. Prior
to withdrawal of the needle negative pressure must be released
to prevent suction of the material into the barrel of the syringe
when the needle exits the skin. Back to
Fine Needle Aspiration Collection |
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