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Vitreous Fluid

Test indication: Detection and characterization of malignant cells in the eye. In cytology, vitreous fluid is most commonly evaluated to detect and characterize malignancy which may have gained access to the eye. Vitreous fluid specimens require special collection procedures under the direction of an ophthalmologist. In addition, due to lack of nutrients in this fluid, cells may rapidly degenerate rendering morphologic evaluation less than optimal if adequate care is not taken.

Methodology: Routine cytopathologic evaluation.
Performed: Monday-Friday.
Reported: 1 day.

Specimen required: For vitreous fluids, there is no minimum amount, but collect as much as possible.

Supplies: Standard vitreous aspiration equipment, a clean collection container for vitreous fluid, a fresh specimen is preferred, (Fixative 50% ethanol if delay transport to laboratory is greater than 24 hour).

Collection procedure: Using standard vitreous collection procedures, collect an appropriate amount of vitreous fluid. Label the specimen with patient’s first and last name, date of birth and specimen source. Submit the specimen to the Cytopathology Laboratory along with the completed Cytology request form.

Note: Specimens submitted for immunocytochemical testing must be submitted fresh. If necessary, on-call personnel can be utilized during the evening or on weekends for processing of urgent specimens or those which need to be processed rapidly to avoid degeneration.Fixation: A fresh specimen is preferred, if a longer time period between collection and processing is anticipated, the specimen may be preserved by adding an equal volume of 50% ethanol to the specimen.

Specimen stability: Refrigerated: 3 days.

Reference interval: By report.

CPT code(s): 88108 concentration and interpretation or 88112 Selective cellular enhancement with interpretation and/or 88106 Cytopathology, filter. Additional CPT codes may apply if special studies are required

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